TABLE 3.
Analyses of asymptomatic leaf samples from nurseries and orchards based on real-time PCR of washed leaf tissues without DNA extraction
| Host(s)a | Cultivar or rootstock | No. of washed samples positive by real-time PCR/total no. of washed samples analyzed | CT rangee | No. of positive isolation samples/total no. of isolation samples analyzedb |
|---|---|---|---|---|
| Prunus amygdalus | Unknown | 1/10 | 33.13 | 1/1 |
| Prunus persica | Unknown | 4/13 | 31.34-40.49 | 0/1 |
| Prunus mahaleb | Santa Lucía SL-64 | 16/55 | 31.24-40.98 | 7/13 |
| P. persica × P. davidiana | Barrier | 11/36 | 23.58-39.39 | 3/4 |
| Prunus amygdalus | Variousc | 0/63 | — | ND |
| Prunus avium | Unknown | 0/6 | — | ND |
| Prunus persica, P. persica var. nectarina, P. persica var. platycarpa | Variousd | 1/42 | 33.76 | ND |
| Prunus amygdalus × P. persica | GxN-15 Garnem | 0/16 | — | ND |
| Prunus cerasifera | Myrobalan | 0/1 | — | ND |
| Prunus insititia | Adesoto | 0/23 | — | ND |
| Prunus persica × P. amygdalus | GF-677 | 0/15 | — | ND |
| Total | 33/280 | 23.58-40.98 | 11/19 |
a
Samples were collected from June to October.
b
Isolation was achieved from samples testing positive by real-time PCR analysis with CT values ranging from 27.69 to 40.98. ND, not determined.
c
Prunus amygdalus cv. Guara, Ferragnes, Ferraduel, Largueta, and Marcona and other unknown cultivars.
d
Prunus persica cv. Badia, Big Top, Nectatop, Sweet Cap, Veruela, VP 58, 0286, E 37, SBN, and ASF and other unknown cultivars.
e
—, no fluorescence above the threshold was recorded.