TABLE 5.

Real-time PCR analyses of comminuted buds collected in nurseries and orchards following DNA extraction

Host(s)a	Cultivar or rootstock	No. of positive samples by real-time PCR/total no. of samples analyzed for comminuted DNA extraction	CT range	No. of positive isolation samples/total no. of samples analyzedb
Prunus amygdalus	Variousc	9/15	21.33-38.03	5/14
Prunus persica	Variousd	11/43	31.24-39.47	2/31
Prunus cerasifera	Myrobalan	3/31	30.86-33.63	2/3
Prunus amygdalus	Variousc	2/99	31.54-32.51	ND
Prunus persica	Variousd	1/33	41.62	ND
Prunus armeniaca	Moniquí	0/3	—f	ND
Prunus amygdalus × P. persica	GxN	0/5	—	ND
Prunus persica × P. amygdalus	GF-677	0/26	—	ND
Prunus salicina, Prunus domestica	Variouse	0/30	—	ND
Total		26/285	21.33-41.62	9/48

^aSamples were collected from January to March.

^bIsolation was achieved for samples testing positive by real-time PCR with C_T values ranging from 21.33 to 33.70. Isolation was not achieved for 25 samples that tested negative by PCR. ND, not determined.

^cPrunus amygdalus cv. Achaak 506, AL-VT 363, Argentina, Aspirilla 547, Ayles, Barter 189, Biota 530, Bulbuente 549, Castañeda 368, Constantí, Coop Mañan 550, Cosa Trova 320, Ferralise 349, Filipo Ceo 360, Forastero, Kata 514, Largueta, Largueta VT 366, Marcona, Marinada, Merino, Pau 234, Picantilli 106, Rana 259, Rachele 258, Spilo 517, Taitona 242, Tarragonés, Texas, Tree 516, Truoito, Vayro, and Vivot 241 and other unknown cultivars.

^dPrunus persica cv. Amarillo Octubre, Baby Gold 5, Baladin VT, Calabacero, Calante, Campillo Rocho, Catherine, Evaisa, Gallur, Jeromo, Jerónimo, Jesca, Maluenda 2375, Manolito, Maruja Tejar, Michelini, Miraflores, Montaced, Montamar, Paraguayo Jota, Red Robin, Roig de Aiotona, Rojo-Amarillo Septiembre, Rojo del Rito, Roza, San Jaime, San Lorenzo, Sudanell 2211, Sudanell 2213, Sudanell 2349, Summer Grand, and Zaragozano and other unknown cultivars.

^ePrunus salicina cv. Golden Japan and other unknown cultivars.

^f—, no fluorescence above the threshold was recorded.