TABLE 2.
Comparison of microarray and RT-qPCR dataa
| Gene | Locus tag | Primer sequences |
Ratio of expression at indicated time as determined by: |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Transcriptome analysis |
RT-qPCR |
||||||||
| Forward | Reverse | 8 h | 1 day | 7 days | 8 h | 1 day | 7 days | ||
| bcaT | L0086 | GTTTGCTTTCACCATTGTTTAACT | ATTAAAAGCCTATCGAACAAAGGAT | 1.1 | 0.8 | 0.4 | 4.2 | 1.2 | 4.6E−02 |
| codY | L0243 | GTCCATTAGCAATCTTCCCAGA | CCACATGATAAATGTTCCCAAAC | 1.1 | 1.1 | 1.0 | 1.5 | 1.8 | 1.5 |
| serA | L0084 | CCACGATTATGAATTTTCCAAAG | CTACAATCCCTTTACTGGCACAC | 1.4 | 0.9 | 0.7 | 3.6 | 0.6 | 0.3 |
| cysK | L0089 | GAGGTTCAGTTAAAGACCGGATT | CTGTGTTCCCAGAGGTAGGTTC | 1.2 | 1.0 | 3.0 | 16.6 | 14.9 | 20.1 |
| gltD | L114827 | AGTCATTGGTGGTGGAGATACAG | CAGTGGGTAAACTTGGTGTGATT | 1.2 | 0.3 | 0.3 | 2.5 | 0.1 | 0.1 |
| lacC | L0032 | CAAAGATTGCTTCTAGTTCTTCTCG | GTGAAGATTTCTATGAGCGTTTGAT | 2.6 | 2.2 | 1.5 | 2.7 | 1.1 | 3.3 |
| gapA | L0004 | GTCTTGTTTTGCCTGAACTTTCT | TCGTCAACTGTAACGTGTTTTTC | 1.4 | 3.2 | 3.6 | 2.4 | 26.0 | 96.3 |
| gapB | L0005 | AACTCAAGGTCGTTTTGATGGTA | GTTAGCTGGGTTAGATTCAGCAG | 1.1 | 1.6 | 1.8 | 2.1 | 3.2 | 3.8 |
| pdhB | L0034 | CAAGCAAAATATGGAGAAGAACG | ATAATTGGGTGGAAACCTTGTGT | 1.7 | 1.9 | 2.4 | 1.3 | 7.3 | 3.6 |
| aldB | L0321 | TTTCACAAAATCAACCCGAATAC | GGAGATGATAACCAGCAACACTC | 1.4 | 1.2 | 1.1 | 4.7 | 2.7 | 2.6 |
| butA | L118271 | GGTAAAGATGATGAATGGGGAAT | GGCCAGCTAAGAATGAAACAAC | 1.5 | 2.9 | 2.3 | 5.0 | 3.8 | 11.3 |
| noxE | L196579 | ATTTCCTGCAATTATTTCACTCTTG | AATCGGCCTAGAAGTTTCATTTAGT | 0.9 | 2.2 | 3.2 | 1.0 | 9.4 | 7.6 |
| murF | L141766 | ATATAACGCGACGAATGCTATGT | CGATTTCTGGTCAGCTCTACTTC | 0.8 | 0.8 | 0.9 | 1.8 | 1.4 | 1.3 |
| dnaK | L178206 | TGGTGTCTTCACAAAATTGATTG | TGAATATCTACAGCGGGTTGATT | 1.8 | 1.6 | 3.8 | 3.6 | 2.3 | 33.6 |
| chiA | L9964 | GACTTGGTCAGTAAATTGGGATG | GGTTGATTGTTAAAGAGCATTGG | 1.2 | 1.8 | 1.4 | 2.7 | 4.9 | 37.9 |
| pepN | L102360 | TTTCCTCGATATTGACCGTAAAA | AAGTGTAAACCTTTGGCATGAAA | 1.3 | 0.9 | 0.5 | 1.8 | 1.1 | 0.5 |
| gyrB | L0283 | GGATGGAATTGCTGTTGAAGTAG | CTAAAACCTTGCTCATGTGTTCC | ||||||
| pi139 | L69762 | GGTTTGCATCTAAAACAAGAACG | TTGGACATTATCAATCACAGCAG | ||||||
| pi302 | L14521 | AATTCATCAAATTTCGGTTTAAGA | AAAAAGTAAGCTAGCATGAATGTG | ||||||
a
RT-qPCR, quantitative reverse transcription-PCR.