FIG. 1.

Functional dissection of TrwC and its target oriT sequence. (a) Map of the TrwC protein showing its functional domains. Tra, transfer (functionality in conjugation); Rel, in vitro relaxase activity; Rec, in vivo site-specific recombinase activity; Hel, DNA helicase activity; Dim, dimerization ability. The data were drawn from references 7 and 32. (b) DNA sequence of the central R388 oriT region (coordinates 201 to 173 from reference 27). The inverted repeat IR₂ is indicated with arrows. Horizontal bars show the minimal sequence requirements for different TrwC activities. bind, in vitro TrwC binding on oligonucleotides; nic, in vitro TrwC nicking and strand-transfer activity on oligonucleotides; recombination, in vivo TrwC-mediated site-specific recombination; transfer, conjugal mobilization (data were drawn from references 7 and 33).