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. 2010 Oct 29;77(1):201–210. doi: 10.1128/AEM.01371-10

FIG. 5.

FIG. 5.

Yeast nuclear import/export assays. (a) Scheme of the yeast nuclear import assay. The test protein (green circle) is fused to transcriptional regulators, which turn on reporter genes if driven into the nucleus, thus allowing the expression of HIS3 and lacZ. Selection is illustrated in the right panels. HIS3 expression allows growth in histidine-deficient medium (−His), and lacZ is monitored by β-galactosidase activity in cells transferred to nitrocellulose filters with X-Gal (NC+X-Gal). (b) Results of the nuclear import (left two panels) and export (right two panels) assay with TrwC and derivatives. The numbers on the plates in panels a and b represent the following: 1, pNIA:VirD2; 2, pNIA:E2; 3, pNIA:N293; 4, pNIA:C774; 5, pNIA:TrwC; 6, pNIA:N600; 7, pNEA:VirE2; 8, pNEA:TrwC; and 9, pNEA:C774.