TABLE 1.
Published plasmids used in this study
| Plasmid | Descriptionb | Reference |
|---|---|---|
| pCEFL | Eukaryotic expression vector | 43 |
| pCIG1028 | oriT1-oriT2 recombination substrate | 7 |
| pCIG1030 | As pCIG1028 + trwA | 7 |
| pCIG1051 | pET3a::trwC(N450) | 7 |
| pCIG1073 | As pCIG1028 with oriT1(173-190) | 7 |
| pCIG1099 | pET3a::trwC(N600) | 7 |
| pCMS13 | pKK223-3::oriT(63-330)muta | 7 |
| pET:trwA | pET3a::trwA | 7 |
| pET3:trwAC | pET3a:PtrwA-trwA-trwC | 10 |
| pET29:trwAC | pET29c:PtrwA-trwA-trwC | 10 |
| pNEA3b | lexA-SV40 NLS-gal4AD fusion | 36 |
| pNEA:VirE2 | Control − for nuclear export | 36 |
| pNIA3b | lexA-gal4AD fusion | 36 |
| pNIA:VirD2 | Control + for nuclear import | 36 |
| pNIA:VirE2 | Control − for nuclear import | 36 |
| pSU19 | Cloning vector | 1 |
| pSU1445 | R388:Tn5tac1 in trwC | 28 |
| pSU1483 | pKK223-3::trwC | 15 |
| pSU1534 | pHG327::trwC(C774) | 32 |
| pSU1600 | pET3a::trwC(N293) | 7 |
| pSU1621 | pET3a::trwC | 17 |
a
Mutation affecting the nic site (TCT/A-to-GAG/A change).
b
SV40 NLS, NLS of simian virus 40.