TABLE 3.
Transfer frequencies of R388 trwC mutant pSU1445 when complemented by different TrwC derivativesa
| Complementation type and plasmid | TrwC protein | Transfer frequencyb |
|---|---|---|
| Complementation by TrwC fusion proteins | ||
| pSU1483 | TrwC | 1.0 × 10−1 |
| pCMS9 | LexA-Gal4AD-TrwC | 4.0 × 10−1 |
| pCMS15 | LexA-NLS-Gal4AD-TrwC | 6.0 × 10−1 |
| pCMS5 | LexA-Gal4AD-TrwC-NLS | 4.0 × 10−1 |
| pMTX720 | LexA-Gal4AD-NLSx2-TrwC | 1.1 × 100 |
| pMTX726 | LexA-Gal4AD-NLS-TrwC | 1.3 × 100 |
| pLA44 | LexA-Gal4AD-TrwC mut | 1.3 × 10−1 |
| pNIA3b | None | <10−7 |
| Relaxase-helicase intragenic complementation | ||
| pCIG1086+pSU1534 | TrwC + C774 | 2.1 × 10−1 |
| pLA35+pSU1534 | N293 + C774 | 1.1 × 10−5 |
| pCIG1070+pSU1534 | N450 + C774 | 1.1 × 10−5 |
| pCIG1103+pSU1534 | N600 + C774 | 3.1 × 10−5 |
| pSU1534 | C774 | <10−7 |
a
Matings were performed as explained in Materials and Methods. Donor strains carried plasmid pSU1445 (R388 TrwC−) and the plasmid(s) indicated in the first column, which code for the TrwC derivatives indicated in the second column.
b
Transfer frequencies are expressed as the number of transconjugants per donor cell. The results represent the means of three to nine independent experiments.