TABLE 5.
Volume back-calculations for average final sample volumes in UF concentrates, secondary concentrates, and total viral NA extracts
| Sample type | n | Total sample vol (liters) | Avg (range) concentrate vol (ml) | Total sample vol (ml)/avg concentrate vol (ml) | Avg total secondary concentrate vol (ml)a | Avg total sample vol (ml)/secondary concentrate vol (liters) | Avg total viral NA extract vol (μl)b | Avg total sample vol (ml) calculated for 5 μl of NA extract |
|---|---|---|---|---|---|---|---|---|
| GW | 10 | 100 | 286 (80-550) | 465 | 0.39 | 96 | 94 | 836 |
| SW | 11 | 100 | 380 (131-1,160) | 373 | 3.1 | 19 | 93 | 201 |
a
Total secondary concentrate from 70 ml of UF concentrate, except for one GW UF concentrate, for which a 35-ml volume was used.
b
Total viral NA extract from 200 μl of secondary concentrate, except for five GW secondary concentrates, for which volumes of 135, 193, 195, 117, and 132 μl were used.