TABLE 3.
Expression yield and biochemical characterization of P. anserina hemicellulolytic CAZymes
| CAZyme | Expression yield (mg/liter)b | Molecular mass (kDa) |
pI |
Apparent optimum pH and tempa |
|||
|---|---|---|---|---|---|---|---|
| Theoretical | Experimentalc | Theoretical | Experimentald | pH | Temp (°C) | ||
| PaMan5A | 440 | 39.4 | 46 | 7.8 | 7.5 | 3-6 (4) | 50-65 (60) |
| PaMan26A | 100 | 49.8 | 62 | 4.7 | 4.3 | 4-6 (4.5) | 40 (40) |
| PaXyn11A | 30 | 28.5 | 41 | 8.3 | 6.2-6.3 | 5-7 (6) | 50-60 (50) |
| PaAbf51A | 90 | 71.7 | 86 | 6.4 | 6.1 | 4-7 (4.5) | 5-75 (70) |
| PaAbf62A | 300 | 37.7 | 48 | 4.8 | 4.6 | 4-6 (4) | 50-60 (55) |
a
Values indicate that the relative enzyme activity remaining was above 50%. Values in parentheses indicate the apparent optimum pH and temperature under the conditions used.
b
Expression yield is expressed in mg of recombinant protein purified from 1 liter of culture.
c
Estimated from SDS-PAGE.
d
Estimated from an IEF gel.