TABLE 4.
Enzymatic properties of P. anserina CAZymes acting on hemicellulases from different sources
| CAZyme | Activity | Substrate | Enzymatic property |
|||
|---|---|---|---|---|---|---|
| SAa ± SEM (U mg−1) | Kmb ± SEM (mg ml−1 or mM) | Vmax ± SEM (U mg−1) | kcat/Kmc (mg−1 min−1 ml or M−1 min−1) | |||
| PaMan5A | Endo-β-1,4-mannanase | Konjac glucomannan | 45 ± 0.9 | 0.7 ± 0.1 | 56.4 ± 1.7 | 3,142 |
| Carob galactomannan | 65 ± 1.4 | 1.7 ± 0.1 | 75.5 ± 1.3 | 1,732 | ||
| LBG galactomannan | 68 ± 3.2 | 4.7 ± 1.4 | 107 ± 14 | 899 | ||
| Ivory nut mannan | 35 ± 1.8 | 1.6 ± 0.4 | 42.3 ± 2.4 | 1,044 | ||
| PaMan26A | Endo-β-1,4-mannanase | Konjac glucomannan | 56 ± 1.9 | 0.8 ± 0.0 | 74.2 ± 1.8 | 5,750 |
| Carob galactomannan | 60 ± 3.4 | 2.6 ± 0.6 | 79.5 ± 5.8 | 1,895 | ||
| LBG galactomannan | 86 ± 4.1 | 5.5 ± 1.2 | 126 ± 13 | 904 | ||
| Ivory nut mannan | 46 ± 2.7 | 1.0 ± 0.1 | 48.8 ± 1.7 | 1,928 | ||
| PaXyn11A | Endo-β-1,4-xylanase | Wheat arabinoxylan | 249 ± 7.4 | 5.8 ± 0.4 | 405 ± 12 | 2,443 |
| Birchwood xylan | 95 ± 4.2 | 3.7 ± 0.5 | 124 ± 5 | 1,172 | ||
| PaAbf51A | α-l-Arabinofuranosidase | pNP-Ara | 22.8 ± 0.6 | 1.2 ± 0.1 | 26.3 ± 0.5 | 1,571 |
| Wheat arabinoxylan | 6.9 ± 0.4 | NDd | ND | ND | ||
| Sugar beet arabinan | 3.1 ± 0.3 | ND | ND | ND | ||
| Debranched arabinan | 0.9 ± 0.0 | ND | ND | ND | ||
| PaAbf62A | α-l-Arabinofuranosidase | pNP-ara | 0.38 ± 0.01 | 6.1 ± 0.3 | 0.61 ± 0.02 | 3.7 |
| Wheat arabinoxylan | 1.0 ± 0.1 | ND | ND | ND | ||
| Sugar beet arabinan | 0.11 ± 0.02 | ND | ND | ND | ||
| Debranched arabinan | 0.22 ± 0.03 | ND | ND | ND | ||
a
SA was determined at 10 mg ml−1 for polysaccharides and at 10 mM for pNP sugars at 40°C and pH 5 in McIlvaine's buffer.
b
Units are in mg ml−1 for polysaccharides and in mM for pNP substrates.
c
Units are in mg−1 min−1 ml for polysaccharides and in mM−1 min−1 for pNP substrates.
d
ND, not detected. Although enzyme activity was present, it was too low to measure the individual kinetic constant.