TABLE 5.
Binding parameters of P. anserina PaMan26A
| Condition | Binding parametera |
|
|---|---|---|
| Kd (μM) | Bmax (mg bound per g of substrate) | |
| Insoluble wheat xylan | 1.85 | 12 |
| Ivory nut mannan | 6.25 | 19 |
| Avicel PH101 | NB | NB |
The binding parameters were determined in triplicate, and the Kd and Bmax values were calculated using double-reciprocal plots. NB, no binding.