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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cell Stem Cell. 2010 Dec 3;7(6):682–693. doi: 10.1016/j.stem.2010.11.013

Figure 3. Bmi-1 regulates the depletion and expansion of self-renewing cells in prostate spheres.

Figure 3

(A) p63 and DAPI stains of scrambled (left) and Bmi-1 shRNA (right) infected spheres. (B) Percentage of p63+ cells in 12 representative scrambled and Bmi-1 shRNA infected spheres. (C) p63 and DAPI stains of GFP (left column) and Bmi-1 (right column) infected p53−/− prostate spheres. (D) Percentage of p63+ cells in 12 representative GFP and Bmi-1 infected spheres. (E) Number of daughter spheres formed from single dissociated scrambled and Bmi-1 shRNA infected and (G) GFP and Bmi-1 infected spheres. (F) Merged images of representative daughter spheres formed from Bmi-1 shRNA (red) infected and (H) Bmi-1-GFP (green) infected spheres. Data represented as mean +/− SEM. Bar=200μm. See also Figures S3, S4, and Table S1.