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SDS-PAGE (Coomassie blue colloidal stain) analysis. (A) Molecular mass marker (Precision Plus Protein standards; Bio-Rad). (B and C) rTbpA (B) and rTbpB (C) eluted from nickel affinity columns. (D) Native proteins (1 to 9) with affinity to porcine transferrin eluted from a CNBr-transferrin affinity column. These nine proteins were further identified by MALDI-TOF/TOF mass spectrometry (see Table 1).
