Skip to main content
. 2010 Nov 10;18(1):43–49. doi: 10.1128/CVI.00292-10

FIG. 2.

FIG. 2.

Polyclonal rabbit serum with goat anti-rabbit IgG-Alexa Fluor 647 (GAR-AF647) strongly stained the mycobacterial surface. We stained mycobacterial surface antigens with anti-BCG rabbit serum and GAR IgG-AF647. (A) GAR IgG-AF647 strongly labeled the bacterial surface. “Pre” indicates preimmune rabbit serum (1:5,000 dilution), and “PI-5” indicates rabbit immune serum (1:5,000 dilution). (B) In addition, rabbit anti-SIINFEKL serum strongly stained M. smegmatis/pMP10 conjugated with GAR IgG-AF647. “M. smeg-pMP10” is the M. smegmatis mc2155 strain expressing the ovalbumin SIINFEKL epitope, and “M. smeg” indicates empty M. smegmatis mc2155. “NRS” indicates normal rabbit serum.