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. 2011 Jan;10(1):54–62. doi: 10.1128/EC.00281-10

Fig. 2.

Fig. 2.

Analysis of allelic polymorphisms on chromosome R of strain SC5314 and mutant derivatives. (A) Location of analyzed polymorphic genes on chromosome R. The position of the centromere (CENR) is also shown. (B) Southern hybridization of ClaI-digested genomic DNA of the indicated strains with a SAP2-specific probe. The identities of the hybridizing fragments are given. SC5314 (wild type), SAP2MS4A and SAP2MS4B (sap2-1Δ/sap2-2Δ), SAP456MS4A and SAP456MS4B (sap4Δ/sap4Δ sap5Δ/sap5Δ sap6Δ/sap6Δ), and DSY459 (sap4Δ/sap4Δ sap5Δ/sap5Δ sap6Δ/sap6Δ) are shown. (C and D) Southern hybridization analysis of HindIII (C)- and EcoRI (D)-digested genomic DNA of strains SC5314 and DSY459 with OPT1- and OPT4-specific probes, respectively. The fragments corresponding to the polymorphic OPT1 and OPT4 alleles of strain SC5314 are indicated.