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Binding of polyclonal phage antibodies to adult buffy coat WBCs and total fetal liver cells. After the second round of selection, polyclonal phage were prepared and analyzed for their ability to bind either adult buffy coat WBCs (B) or total fetal liver (D). There is a large shift in the fetal liver cells compared with a minimal shift of a small population of adult buffy coat WBCs. In contrast, control wild-type fd phage did not significantly shift either cell population (A and C).
