Figure 2.

Binding of monoclonal phage antibodies to adult buffy coat WBCs and total fetal liver cells. Phage were prepared from individual colonies after the second round of selection and analyzed for their ability to bind adult buffy coat WBCs or total fetal liver cells. The representative antibody fd-H7 (B and E) bound only fetal liver cells, whereas the antibody fd-B6 (C and F) bound fetal liver and a small subpopulation of WBCs consistent with monocytes. Staining with wild-type fd phage (A and D) is included as a control.