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Staining of total fetal liver by a fetal NRBC specific phage antibody. Fetal liver cells were incubated with biotinylated phage antibody FSG9, applied to microscope slides, and then stained with FITC anti-fetal hemoglobin antibody (A), streptavidin Alexa fluor 546 to detect phage binding (B), or 4′,6-diamidino-2-phenylindole (D). Fetal hemoglobin and phage binding were visualized together with a dual band filter (C). Only fetal NRBCs stained with both. r, enucleated RBC; w, WBC.
