Fig. 2.

hph1Δ hph2Δ cells are sensitive to growth under conditions of high concentrations of heavy metals (ZnCl₂, CdCl₂, CoCl₂, and CsCl₂), CaCl₂, oxidative stress (H₂O₂), and a nonfermentable carbon source (ethanol). hph1Δ cells are sensitive to growth on ZnCl₂. Wild-type (BY4741), hph1Δ (number 1621), hph2Δ (number 380), and hph1Δ hph2Δ (VHY60) strains were grown to stationary phase in YPD. The cultures were diluted to 2.5 × 10⁶ cells/ml, and 7-fold serial dilutions were plated on YPD plates containing the indicated additions. The plates were incubated at room temperature for 2 days (YPD, pH 7.6, CaCl₂, and YP-ethanol), 4 days (ZnCl₂, CdCl₂, CoCl₂, CsCl₂, and H₂O₂₎, and 5 days (NaCl and LiCl).