Table 1.
Strains used in this study
| Strain | Genotype | Reference |
|---|---|---|
| BY4741 (YSC1048) | MATaleu2Δ ura3Δ his3Δ met15Δ | Yeast deletion collection (Open Biosystems) |
| BY4742 (YSC1049) | MATα leu2Δ ura3Δ his3Δ lys2Δ | Yeast deletion collection (Open Biosystems) |
| VHY70 | MATaleu2Δ ura3Δ his3Δ | 24 |
| VHY60 | MATaleu2Δ ura3Δ his3Δ met15Δ hph1Δ::KanMX4 hph2Δ::KanMX4 | 24 |
| hph1Δ (no. 1621) | Isogenic to BY4741 | Yeast deletion collection (Open Biosystems) |
| hph2Δ (no. 380) | Isogenic to BY4741 | Yeast deletion collection (Open Biosystems) |
| pkr1Δ (no. 6564) | Isogenic to BY4741 | Yeast deletion collection (Open Biosystems) |
| sec71Δ (no. 3311) | Isogenic to BY4741 | Yeast deletion collection (Open Biosystems) |
| hph1Δ hph2Δ sec71Δ | MATaleu2Δ ura3Δ his3Δ hph1Δ::KanMX4 hph2Δ::KanMX4 sec71Δ::KanMX4 | This study |
| hph1Δ hph2Δ pkr1Δ | MATa leu2Δ ura3Δ his3Δ hph1Δ::KanMX4 hph2Δ::KanMX4 pkr1Δ::KanMX4 | This study |
| vma21Δ (no. 4735) | Isogenic to BY4741 | Yeast deletion collection (Open Biosystems) |
| BY4743-SEC71-TAP | MATa/α leu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lys2Δ/LYS2 SEC71::TAP::HIS3MX6/SEC71 | This study |
| BY4743-SEC72-TAP | MATa/α leu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lys2Δ/LYS2 SEC72::TAP::HIS3MX6/SEC72 | This study |
| BY4743-SEC61-TAP | MATa/α leu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lys2Δ/LYS2 SEC61::TAP::HIS3MX6/SEC61 | This study |
| BY4743-SEC62-TAP | MATa/α leu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lysΔ2/LYS2 SEC62::TAP::HIS3MX6/SEC62 | This study |
| BY4743-SEC63-TAP | MATa/α leu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lysΔ2/LYS2 SEC63::TAP::HIS3MX6/SEC63 | This study |