Fig. 5.

Titration curves for glycosylated N-Flo1p with increasing concentrations of dimannoses. The spheres show the change in fluorescence intensity at each concentration of dimannose, ranging from 10 μM to 100 mM for α(1,2)-dimannose and from 10 μM to 90 mM for α(1,3)-dimannose and α(1,6)-dimannose. Determination of the K_D values was performed by fitting (full line) the experimental data points with the binding model. (a) K_{D, Ha} = 205.70 μM and K_{D, La} = 8.47 mM for α(1,2)-dimannose. (b) K_{D, Ha} = 149.50 μM and K_{D, La} = 10.99 mM for α(1,3)-dimannose. (c) K_{D, Ha} = 148.20 μM and K_{D, La} = 17.55 mM for α(1,6)-dimannose.