TABLE 3.
1H- and 13C-NMR parameters established from COSY, NOESY, and HSQCa
| Product | H | Shift (ppm) | J coupling (Hz) | C | Shift (ppm) |
|---|---|---|---|---|---|
| GlcNAcα-PP-(CH2)11-OPh | |||||
| GlcNAc | H-1 | 5.53 (5.64) | C-1 | 96 | |
| H-2 | 3.97 | C-2 | 55 | ||
| H-3 | 3.75 | C-3 | 72.9 | ||
| H-4 | 3.33 (3.45) | C-4 | 71.9 | ||
| GlcNAcα-PP-decanol (7) | |||||
| GlcNAc | H-1 | 5.45 | 3.2, 7.2 | C-1 | 94.5 |
| Galβ1-4GlcNAc-PP-(CH2)11-OPh (WfeD) | |||||
| GlcNAc | H-1 | 5.561 | C-1 | 94.7 | |
| H-2 | 4.027 | C-2 | 53.1 | ||
| H-3 | 3.846 | C-3 | 69.4 | ||
| H-4 | 3.626 | C-4 | 79.1 | ||
| Gal | H-1 | 4.345 | 7.6 | C-1 | 103.4 |
| H-2 | 3.538 | 6.9, 10.1 | C-2 | 70.8 | |
| H-3 | 3.465 | 2.9, 9.5 | C-3 | 73.1 | |
| H-4 | 3.792 | 2.5 | C-4 | 68.8 | |
| Galβ1-3GlcNAc-PP-(CH2)11-OPh (17) | |||||
| GlcNAc | H-1 | 5.58 | C-1 | 94.8 | |
| H-2 | 4.14 | C-2 | 52.4 | ||
| H-3 | 3.84 | C-3 | 80.3 | ||
| Gal | H-1 | 4.35 | C-1 | 104.0 | |
| Glcβ1-3GlcNAc-PP-(CH2)11-OPh (6) | |||||
| GlcNAc | H-1 | 5.54 | C-1 | 95.0 | |
| H-2 | 4.14 | C-2 | 54.0 | ||
| H-3 | 3.87 | C-3 | 82.8 | ||
| H-4 | 3.42 | C-4 | 70.3 | ||
| Glc | H-1 | 4.41 | C-1 | 105.0 | |
| H-2 | 3.18 | C-2 | 74.5 | ||
| H-3 | 3.37 | C-3 | 77.6 | ||
| H-4 | 3.33 |
a
Shown is a comparison of NMR parameters between substrates and β3-GalT, β4-GalT, and β3-Glc-transferase enzyme products. NOESY, nuclear Overhauser enhancement spectroscopy.