FIG. 3.

ChxR is associated with its own promoter in vivo. To determine whether ChxR recognizes the chxR promoter during a chlamydial infection, ChxR was cross-linked to DNA, using formaldehyde, at 36 hpi and immunoprecipitated from the lysates using antibodies that recognize ChxR (αChxR). PCR was then performed with primers specific for the chxR promoter. The lack of a PCR product with primers to the CT863 promoter supports that ChxR specifically recognizes the chxR promoter in vivo. The presence (+) or absence (−) of C. trachomatis genomic DNA (DNA) was used as PCR controls for both promoters.