FIG. 6.

Mutations within the DNA recognition motif significantly reduce ChxR-DNA interaction. (A) The DR2 nucleotide sequence from the chxR promoter is shown. The two consensus recognition sequences are underlined, and the boldface nucleotides indicate the sites of triple-cytosine mutations. (B) EMSAs were performed with increasing concentrations of ChxR (39 nM to 3.9 μM) and 39 nM concentrations of each DNA construct: wild-type sequence (WT), DR2-2 mutant, DR2-1 mutant, and DR2-1/2 double mutant.