FIG. 7.

Single-base-pair contributions to ChxR-DNA interactions within the DR2 binding site. EMSAs were performed with 5 μM ChxR and 50 nM DNA containing transversion mutations. The target DNA used in the experiment was the DR2 sequence from the chxR promoter, comprising the DR2 half-sites (underlined). The percentages of DNA shifted with each transversion mutation (n = 3) are shown in the graph relative to the DNA shifted with the wild-type sequence (n = 18). The amount of DNA shifted was quantified using the photon emission of the SYBR green at 520 nm. The mutations that resulted in a significant (P < 0.05) reduction of DNA-interaction are denoted by an asterisk.