TABLE 3.

Metabolite excretion of S. meliloti argC complemented strains under the S. meliloti pspeB promoter

Strain	Metabolite excretion (mean ± SD)a
	MM succinate-ammonium (8 h)				MM mannose-nitrate (24 h)
	Glu*	Gly*	α-Kg†	Succ†	Glu*	Gly*	α-Kg†
S. meliloti
1021 wild type	1.6 ± 0.7	0.1 ± 0.1	13.6 ± 1.0	226.6 ± 25.2	0.1 ± 0.2	12.6 ± 2.0	10.4 ± 0.8
argC mutant	7.1 ± 3.3	1.5 ± 0.1	228.6 ± 74.0	3356.6 ± 1201.2	24.6 ± 0.5	396.4 ± 78.2	129.2 ± 36.3
argC mutant (+1 mM Arg)	0.2 ± 0.1	0.4 ± 0.2	11.7 ± 0.7	176.8 ± 1.2	0.4 ± 0.3	3.6 ± 0.8	9.2 ± 4.8
argC mutant complemented strains
pFGP16 (S. meliloti)	1.0 ± 0.2	0.8 ± 0.1	13.3 ± 0.9	235.6 ± 23.0	0.1 ± 0.5	54.4 ± 13.0	11.3 ± 1.2
pFGP17 (A. tumefaciens)	6.5 ± 0.3	0.6 ± 0.1	68.5 ± 3.9	1246.0 ± 93.9	11.4 ± 2.4	183.4 ± 74.9	40.6 ± 4.6
pFGP18 (R. etli)	1.1 ± 0.3	0.5 ± 0.1	53.7 ± 3.8	854.8 ± 38.7	0.1 ± 0.2	168.2 ± 17.9	19.7 ± 2.7
pFGP19 (M. loti)	15.9 ± 2.4	1.6 ± 0.4	328.4 ± 11.6	5217.9 ± 1841.0	18.4 ± 0.3	898.0 ± 10.6	151.6 ± 8.4

^aGlu, glutamate; Gly, glycine; α-Kg, α-ketoglutarate; Succ, succinate. *, Expressed as nmol mg of protein⁻¹; †, expressed as μmol mg of protein⁻¹.