TABLE 4.
ArgC and SpeB specific activities of S. meliloti argC complemented strains under the control of different promoters
| Protein and conditions | Mean ArgC and SpeB sp act ± SDa of argC mutant complemented strainsa |
||||
|---|---|---|---|---|---|
| WT | S. meliloti | A. tumefaciens | R. etli | M. loti | |
| ArgC | |||||
| pargC (pFGP12 to pFGP15) | |||||
| Succinate-ammonium | 16.6 ± 0.4 | 134.0 ± 2.6 | 80.1 ± 2.3 | 83.1 ± 1.4 | 13.3 ± 0.8 |
| Mannose-nitrate | 16.3 ± 0.4 | 68.8 ± 0.9 | 28.7 ± 1.4 | 32.3 ± 1.9 | 7.1 ± 0.3 |
| plac (pFGP8 to pFGP11) | |||||
| Succinate-ammonium | 16.7 ± 0.3 | 405.4 ± 3.4 | 49.0 ± 1.5 | 59.5 ± 0.9 | 60.6 ± 0.7 |
| Mannose-nitrate | 16.4 ± 0.1 | 432.4 ± 20.7 | 81.7 ± 2.8 | 32.1 ± 3.1 | 81.9 ± 8.7 |
| pspeB (pFGP16 to pFGP19) | |||||
| Succinate-ammonium | 16.7 ± 0.3 | 20.8 ± 0.4 | 19.3 ± 0.5 | 18.9 ± 1.3 | 0.8 ± 0.2 |
| Mannose-nitrate | 16.1 ± 0.3 | 25.0 ± 0.1 | 16.6 ± 0.1 | 17.7 ± 0.1 | 0.9 ± 0.1 |
| speB-argC (pFGP1 to pFGP4)b | |||||
| Succinate-ammonium | 14.5 ± 0.3 | 102.6 ± 7.4 | 107.5 ± 3.9 | 38.0 ± 6.6 | 0.8 ± 0.2 |
| Mannose-nitrate | 16.2 ± 0.1 | 57.3 ± 10.3 | 115.4 ± 1.7 | 35.2 ± 0.8 | 1.0 ± 0.1 |
| SpeBc | |||||
| speB-argC (pFGP1 to pFGP4) | |||||
| Succinate-ammonium | 6.5 ± 0.1 | 39.9 ± 6.1 | 53.2 ± 2.3 | 55.1 ± 1.9 | 62.2 ± 1.2 |
| Mannose-nitrate | 9.2 ± 0.1 | 46.6 ± 3.4 | 43.7 ± 3.4 | 38.9 ± 1.6 | 21.5 ± 6.6 |
a
Expressed as nmol of NADP min−1 mg of protein−1, except as noted (see footnote c). WT, S. meliloti 1021 wild-type strain.
b
That is, constructs containing speB and argC under their own promoters.
c
Expressed as μg of ammonia mg of protein−1.