TABLE 5.
mRNA, protein quantification, and translational efficiency index for S. meliloti argC mutant complemented strains
| Promoter and parametera |
argC mutant complemented strainsb |
||||
|---|---|---|---|---|---|
| WT | S. meliloti | A. tumefaciens | R. etli | M. loti | |
| pargC (pFGP12 to pFGP15) | |||||
| mRNA | 1 | 9.4 ± 0.5 | 51.1 ± 4.8 | 127.6 ± 22.4 | 19.9 ± 0.4 |
| Proteomic protein | 9.8 ± 0.5 | 771.2 ± 91.1 | 442.7 ± 20.8 | 331.6 ± 29.9 | 273.6 ± 58.8 |
| TEI | 9.8 | 82.0 | 8.7 | 2.6 | 13.8 |
| plac (pFGP8 to pFGP11) | |||||
| mRNA | 1 | 121.4 ± 11.8 | 1,759.2 ± 68.2 | 1,596.7 ± 69.7 | 1,598.7 ± 42.9 |
| Proteomic protein | 9.5 ± 0.1 | 1,834.2 ± 100.3 | 1,399.5 ± 213.2 | 91.7 ± 26.9 | 1,696.1 ± 303.1 |
| TEI | 9.5 | 15.1 | 0.8 | 0.1 | 1.1 |
| pspeB (pFGP16 to pFGP19) | |||||
| mRNA | 1 | 4.9 ± 0.6 | 53.1 ± 2.9 | 35.0 ± 1.1 | 162.9 ± 9.5 |
| Proteomic protein | 9.5 ± 0.1 | 25.1 ± 0.1 | 65.5 ± 2.8 | 72.9 ± 7.9 | 67.4 ± 5.2 |
| TEI | 9.5 | 5.1 | 1.2 | 2.1 | 0.4 |
a
mRNA values were normalized relative to the abundance of mRNA in the wild-type strain. Proteomic protein was quantified by using spectrometry and dot pixels. TEI, translational efficiency index (proteomic protein/mRNA).
b
WT, S. meliloti 1021 wild-type strain. Cells were grown for 8 h in MM supplemented with 10 mM succinic acid and 10 mM ammonium chloride.