TABLE 3.

Relative activation compared with relative binding for activators and mutants at MarA-SoxS-Rob regulon promoters^a

Promoter class and name	WT MarA			MarA(Q91A)			Position 12c	MarA(E89A)			SoxS
	RA	RB	KD (nM)b	RA	RB	KD (nM)		RA	RB	KD (nM)	RA	RB	KD (nM)
Class I
marRAB	1.0	1.0	25	0.9	∼0.8	30	T	1.1	1.0	25	1.1	0.8	30
acnA	1.0	1.0	180	0.1	0.7	250	A	5.4	6.0	30	3.2	3.0	60
acrAB	1.0	1.0	30	1.0	0.6	50	A	1.8	1.5	20	1.8	0.8	40
fpr	1.0	—	>250	0.5	—	>250	G	4.9	∼5.0	50	23.0	>2.0	200
mdtG	1.0	1.0	200	0.6	<0.8	>250	C	4.1	6.7	30	3.7	1.3	150
zwf	1.0	1.0	200	0.9	<0.8	>250	G	2.5	6.7	30	2.3	2.7	75
Class II
fumC	1.0	1.0	50	0.2	<0.3	>150	G	0.5	∼1.7	30	5.3	∼1.0	50
inaA	1.0	—	≫150	0.2	—	≫150	C	1.5	∼3.0	50	0.8	—	≫150
mdaB	1.0	1.0	50	1.3	0.8	63	T	0.6	1.4	35	0.3	0.5	100
micF	1.0	1.0	25	0.2	1.0	25	T	1.0	1.0	25	1.0	∼2.0	50
nfsB	1.0	1.0	200	1.3	∼.8	∼250	C	0.4	2.7	75	0.6	1.3	150
pqiA	1.0	—	≫150	0.0	—	≫150	T	1.9	—	≫150	4.0	—	≫150
sodA	1.0	—	≫150	1.2	>1.0	>150	A	1.5	∼1.0	>150	2.3	—	≫150
ybjC	1.0	—	≫150	0.3	—	≫150	T	1.5	∼1.5	100	0.8	—	≫150
yhbW	1.0	—	>150	0.8	<1.0	≫150	T	0.2	∼1.5	100	0.1	—	>150

^aThe data for the activation relative to that of WT MarA are from Table 2 and Fig. 1. RA, relative activation; RB, relative binding constant. Where values are shown in boldface, the relative activation did not parallel the relative binding. —, value could not be calculated.

^bThe dissociation constant (K_D) was calculated from gel shift assays similar to those shown in Fig. 2.

^cThe nucleotide at position 12 for this marbox sequence.