TABLE 2.
Identification of S. epidermidis proteins whose secretion is inhibited in a dose-dependent fashion by arylomycin C16
| Locus | Protein | Signal peptideb | Spot intensitya |
|||
|---|---|---|---|---|---|---|
| 0× | 0.5× | 1× | 4× | |||
| SERP0318 | SsaA-like | 1MKKLAFAITAASGAAAVLSHHDAEA | +++ | − | − | − |
| SERP0379 | LtaS | 144WLDNRAFSKKFVPVVMATSVALFFLNLAFA | ++++ | +++ | ++ | + |
| SERP0636 | AtlE | 1MAKKFNYKLPSMVALTLFGTAFTAHQANA | +++ | − | − | − |
| SERP1397 | SspA | 1MKKRFLSICTMTIAALATTTMVNTSYA | +++ | ++ | ++ | − |
| SERP1460 | BlaR1 | 302NLKKQSKLILIFICIFTFFIMIIQSQFLMG | +++ | − | − | − |
| SERP1428 | IsaB | 1MKIFKLTSLTLAALTLAFPFSHVAQA | +++ | ++ | + | − |
| SERP2138 | IsaA | 1MKKTVIASTLAVSLGIAGYGLSGHEAHA | ++++ | −/++/++++ | −/+/+++ | −/+/++ |
| SERP2263 | 1MKKNKFLVYLLSTALITPTFATQTAFA | +++ | ++ | − | − | |
| SERP2297 | GehC | 1MKTRQNKYSIRKFSVGASSILIAALLFMGGGSAQA | +++ | − | − | − |
| SERP2388 | GehD | 1MKNNNETRRFSIRKYTVGVVSIITGITIFVSGQHAQA | ++++c | −/++ | −/+ | −c |
| SERP2390 | SspB | 1MKKKLSYMITIMLAFTLSLALGLFFNSAHA | +++c | ++c | +c | +c |
a
Qualitative spot intensity as a function of added arylomycin C16, determined by visual inspection of 2D gels. The intensities for multiple spots are separated by slashes (see text).
b
Numbers correspond to the translated sequence. The sequences of LtaS and BlaR1 were arbitrarily started at residues 144 and 302, respectively.
c
All spots showed roughly the same sensitivity to inhibition.