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Purification of native QscR. Samples were analyzed by SDS-PAGE. Lane 1, molecular weight markers (the molecular mass of each marker in kilodaltons is indicated to the left of the gel); lane 2, cleared cell extract; lane 3, 40% ammonium sulfate precipitate; lane 4, pooled QscR-containing fractions from HiTrap Q HP column chromatography; lane 5, pooled QscR-containing fractions from HiPrep 16/10 heparin FF column chromatography.
