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. 2010 Nov 19;193(2):421–428. doi: 10.1128/JB.01041-10

FIG. 2.

FIG. 2.

An electrophoretic mobility shift assay for binding of purified QscR to a PA1897 promoter fragment. Lane 1, control with no QscR in the reaction mixture; lane 2, 1 nM QscR and no added 3OC12-HSL; lane 3, 5 nM QscR and no added 3OC12-HSL; lane 4, 1 nM QscR plus 5 μM 3OC12-HSL; lane 5, 5 nM QscR plus 5 μM 3OC12-HSL. The positions of nonspecific DNA (N), QscR-free target DNA (F), and QscR-bound DNA (B) are indicated by the arrows to the right of the gel. The promoter fragment is 339 bp, and it includes 301 bp upstream of PA1897.