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. 2010 Oct 18;31(1):30–42. doi: 10.1128/MCB.00648-10

FIG. 3.

FIG. 3.

Nek10 depletion impairs UV-induced MEK1/2 activation. (A) Knockdown of Nek10 in HEK293 cells. HEK293 cells were transfected with pools of esiRNA against Nek10 (top) or dsRed (control), and knockdown was confirmed by semiquantitative RT-PCR (middle). HEK293 cells were cotransfected with pools of esiRNA and Flag-Nek10. Lysates were immunoblotted as indicated (bottom). (B) Knockdown of Nek10 impairs ERK1/2 activation following UV irradiation. HEK293 cells transfected with esiRNA were UV irradiated (250 J/m2) and harvested 30 min later. Lysates were immunoblotted as indicated. (C) Knockdown of Nek10 does not affect mitogenic ERK1/2 activation. HEK293 cells transfected with esiRNA were UV irradiated (20 J/m2) or stimulated with EGF (1 ng/ml) and harvested 30 min later. Lysates were immunoblotted as indicated. (D) Knockdown of Nek10 impairs MEK1/2 activation following UV irradiation. HEK293 cells transfected with esiRNA were UV irradiated (250 J/m2) or stimulated with EGF (0.1 ng/ml) and harvested 30 min later. Lysates were immunoblotted as indicated.