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. 2010 Oct 25;31(1):63–80. doi: 10.1128/MCB.00468-10

FIG. 5.

FIG. 5.

Glomerular lesions and expression of synaptopodin in 13-week-old PI3KC2α-deficient mice. (A and B) Representative glomerular lesions in H&E-stained kidney sections from KO (−/−) mice, showing diffuse mesangial sclerosis. (B) On the left, there is a shrunken nonfunctional sclerotic glomerulus. On the right, there is segmental mesangial sclerosis involving most of the glomerulus; a few patent capillary loops are present, with associated hypertrophic podocytes (arrows). (C to F) PAS-stained kidney sections show normal glomeruli in WT (+/+) mice (C) and glomerular lesions in KO (−/−) mice (D to F) at 13 weeks of age. (D) Lesions varied from relatively mild podocyte hypertrophy and multifocal mesangial thickening (green arrow) to diffuse mesangial sclerosis with ablation of capillaries (yellow arrow). (E and F) Arrows show multilobulated mesangial sclerosis and crescent formation, respectively. (G and H) Synaptopodin expression in kidney sections from WT and KO mice, showing reduced expression in sclerotic juxtaglomerular and cortical glomeruli but linear staining in subcortical glomeruli (arrow). Original magnifications: A, ×20; B, ×40; C to F, ×60; G and H, ×40.