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. 2010 Oct 11;79(1):108–117. doi: 10.1128/IAI.00338-10

FIG. 5.

FIG. 5.

Leukocyte analysis after antibody treatment induced polymorphonuclear leukocyte (PMN) depletion, without or with subsequent inoculation of L. infantum chagasi. (A) Mice were inoculated with 25 μg of either MAb RB6-8C or MAb 1A8 intravenously to deplete neutrophils or with control rat Ig. Total CD45+ leukocytes from peripheral blood were analyzed by flow cytometry for the proportion of Ly6G+ neutrophils either 1 day before or 1 day after depletion. (B) The proportion of Ly6G+ neutrophils in peripheral blood CD45+ leukocytes was quantified at the indicated number of days after inoculation of 25 μg of neutrophil-depleting MAb RB6-8C or control Ig. (C) BALB/c mice were treated with depleting or control Ab as described in panel B, and 24 h later metacyclic L. infantum chagasi promastigotes were inoculated intradermally in the ear. Contour plots show total CD45+ leukocytes or CD45+ leukocytes staining for markers that distinguish different populations of macrophages or dendritic cells. Results show the means ± standard deviations of three mice/group/time point from a representative experiment of three. Two-way ANOVA was performed to test significance. ***, P < 0.001.