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. 2010 Nov 1;79(1):360–368. doi: 10.1128/IAI.00873-10

FIG. 3.

FIG. 3.

Comparison of CIqPCR with the traditional CI. In the CIqPCR assay, a reference strain (the ΔSTM0314 strain) and three deletion strains (ΔSTM2209, ΔSTM3096, and ΔSTM4333 strains) with barcodes were mixed equivalently, and the mix was used to infect a group of three Nramp1+/+ 129SvJ mice at 104 CFU/mouse. For the traditional CI infection, a mixture containing a reference strain (MA6054) and each single strain at a 1:1 ratio was used to infect a group of three Nramp1+/+ 129SvJ mice at 104 CFU/mouse. Mice were sacrificed at days 2, 5, and 7 postinfection, and the intracellular bacteria residing in the spleens were enumerated using the traditional CI formula or CIqPCR formula as described in Materials and Methods. There was no statistically significant difference between the two methods based on Student's t test.