Strains |
|
|
E. coli
|
|
|
HB101 |
F−mcrB mrr hsdS20(rB− mB−)recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20
|
B. Dupuy |
MC135 |
HB101 containing pRK24 and pMC123 |
This study |
MC138 |
HB101 containing pRK24 and pMC125 |
This study |
MC161 |
HB101 containing pRK24 and pMC147 |
This study |
C. difficile
|
|
|
630 |
Clinical isolate |
71 |
JIR8094 |
Erms derivative of strain 630 |
44 |
CCUG37769 |
Serotype A4; ToxA+, ToxB+
|
Lars Burman |
MC119 |
JIR8094 cprK1
|
This study |
MC137 |
JIR8094 pMC123 |
This study |
MC141 |
JIR8094 cprA::ermB
|
This study |
MC146 |
MC141::Tn916 (cprABC); Ermr
|
This study |
MC162 |
JIR8094 pMC147 |
This study |
MC163 |
MC119 pMC147 |
This study |
MC164 |
MC119 pMC123 |
This study |
B. subtilis
|
|
|
BS49 |
CU2189::Tn916
|
P. Mullany |
MC144 |
BS49 Tn916::pMC130 |
This study |
Plasmids |
|
|
pRK24 |
Tra+, Mob+; bla, tet
|
65 |
pCR2.1 |
bla, kan
|
Invitrogen |
pUC19 |
Cloning vector; bla
|
72 |
pCE240 |
C. difficile TargeTron construct based on pJIR750ai (group II intron, ermB::RAM, ltrA); catP
|
C. Ellermeier |
pSMB47 |
Tn916 integrational vector; Cmr, Ermr
|
36 |
pJIR1456 |
E. coli-C. perfringens shuttle vector; catP
|
35 |
pMC122 |
pCE240 retargeted to cprA
|
This study |
pMC123 |
E. coli-C. difficile shuttle vector; bla, catP
|
This study |
pBL58 |
pMC123 with BsrGI and two HindIII sites removed |
This study |
pMC125 |
5.48 kb SfoI/SmaI fragment from pMC122 cloned in SmaI site of pMC123 |
This study |
pMC130 |
2,945-bp DNA sequence corresponding to cprABC plus 500-bp upstream sequence cloned as BamHI/SphI fragment in pSMB47 |
This study |
pMC147 |
pBL58 with 1,798 bp of CD1352 and upstream promoter region (PcprK) |
This study |