TABLE 1.
Bacterial strains and plasmids
| Plasmid or strain | Relevant genotype or features | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| HB101 | F−mcrB mrr hsdS20(rB− mB−)recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 | B. Dupuy |
| MC135 | HB101 containing pRK24 and pMC123 | This study |
| MC138 | HB101 containing pRK24 and pMC125 | This study |
| MC161 | HB101 containing pRK24 and pMC147 | This study |
| C. difficile | ||
| 630 | Clinical isolate | 71 |
| JIR8094 | Erms derivative of strain 630 | 44 |
| CCUG37769 | Serotype A4; ToxA+, ToxB+ | Lars Burman |
| MC119 | JIR8094 cprK1 | This study |
| MC137 | JIR8094 pMC123 | This study |
| MC141 | JIR8094 cprA::ermB | This study |
| MC146 | MC141::Tn916 (cprABC); Ermr | This study |
| MC162 | JIR8094 pMC147 | This study |
| MC163 | MC119 pMC147 | This study |
| MC164 | MC119 pMC123 | This study |
| B. subtilis | ||
| BS49 | CU2189::Tn916 | P. Mullany |
| MC144 | BS49 Tn916::pMC130 | This study |
| Plasmids | ||
| pRK24 | Tra+, Mob+; bla, tet | 65 |
| pCR2.1 | bla, kan | Invitrogen |
| pUC19 | Cloning vector; bla | 72 |
| pCE240 | C. difficile TargeTron construct based on pJIR750ai (group II intron, ermB::RAM, ltrA); catP | C. Ellermeier |
| pSMB47 | Tn916 integrational vector; Cmr, Ermr | 36 |
| pJIR1456 | E. coli-C. perfringens shuttle vector; catP | 35 |
| pMC122 | pCE240 retargeted to cprA | This study |
| pMC123 | E. coli-C. difficile shuttle vector; bla, catP | This study |
| pBL58 | pMC123 with BsrGI and two HindIII sites removed | This study |
| pMC125 | 5.48 kb SfoI/SmaI fragment from pMC122 cloned in SmaI site of pMC123 | This study |
| pMC130 | 2,945-bp DNA sequence corresponding to cprABC plus 500-bp upstream sequence cloned as BamHI/SphI fragment in pSMB47 | This study |
| pMC147 | pBL58 with 1,798 bp of CD1352 and upstream promoter region (PcprK) | This study |