TABLE 2.
Immunodominant Chlamydia antigens recognized by sera from BALB/c mice immunized with live EB and UV-EB
| Protein identifier | Antibody response to the indicated immunization regimen |
Description | Size (aa)a | |||
|---|---|---|---|---|---|---|
| Live EB i.n. | Live EB i.vag. | UV-EB with CpG/Montanide | UV-EB with alum | |||
| TC0001 | + | + | − | − | Delta-aminolevulinic acid dehydratase | 333 |
| TC0004 | − | + | + | − | Transcription elongation protein | 714 |
| TC0012 | + | + | − | − | DNA topoisomerase I | 865 |
| TC0018 | + | + | − | − | 5-Formyltetrahydrofolate cyclo-ligase, putative | 178 |
| TC0031 | + | + | − | + | DNA gyrase, subunit A | 490 |
| TC0035 | + | + | − | − | Conserved hypothetical protein | 824 |
| TC0040 | + | + | + | − | Type III secretion cytoplasm ATPase, SctN | 442 |
| TC0043 | + | + | − | − | Type III secretion translocase, SctQ | 373 |
| TC0045 | + | + | − | − | Type III secretion protein, SctC | 672 |
| TC0047 | + | + | + | − | Conserved hypothetical protein | 177 |
| TC0052 | + | + | + | + | Major outer membrane protein | 387 |
| TC0063 | + | + | − | − | Conserved hypothetical protein | 224 |
| TC0066 | + | + | − | − | Hypothetical protein | 334 |
| TC0077 | + | + | − | − | Poly(A) polymerase family protein | 410 |
| TC0078 | − | + | − | − | ATP-dependent Clp protease, ATP-binding regulatory subunit, ClpX | 419 |
| TC0079 | + | + | − | − | ATP-dependent Clp protease, proteolytic subunit | 203 |
| TC0084 | + | + | + | − | Conserved hypothetical protein | 769 |
| TC0093 | + | + | − | − | NifU-related protein | 260 |
| TC0104 | + | + | − | − | Bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II protein, RibA/rib | 424 |
| TC0114 | + | + | − | + | Hypothetical protein | 122 |
| TC0116 | + | + | − | − | NADH:ubiquinone oxidoreductase, beta subunit, putative | 431 |
| TC0117 | + | + | − | + | Conserved hypothetical protein | 114 |
| TC0133 | + | + | + | + | Translation elongation factor P | 190 |
| TC0136 | + | + | − | + | 60-kDa chaperonin, putative | 513 |
| TC0137 | + | + | − | − | UDP-N-acetylmuramoylalanyl-d-glutamyl-2,6-diaminopimelate-d-alanyl-d-alanyl ligase MurF, putative | 452 |
| TC0140 | + | + | + | + | Conserved hypothetical protein | 243 |
| TC0144 | + | - | − | − | Conserved hypothetical protein | 137 |
| TC0149 | − | + | − | − | Conserved hypothetical protein | 564 |
| TC0151 | + | − | − | − | 3-Oxoacyl-(acyl carrier protein) synthase II, FabF | 418 |
| TC0153 | + | + | + | − | Inorganic pyrophosphatase, putative | 209 |
| TC0160 | − | + | − | − | Conserved hypothetical protein | 250 |
| TC0163 | + | + | + | + | Lysyl-tRNA synthetase | 524 |
| TC0166 | + | + | − | − | Conserved hypothetical protein | 350 |
| TC0177 | + | + | − | − | Conserved hypothetical protein | 164 |
| TC0178 | + | + | − | − | Glycyl-tRNA synthetase, tetrameric type, alpha/beta subunits | 1003 |
| TC0181 | + | + | − | − | Glycogen synthase | 474 |
| TC0188 | + | + | − | + | Hypothetical protein | 47 |
| TC0189 | + | + | − | + | Conserved hypothetical protein | 450 |
| TC0190 | + | + | + | - | Metalloprotease, insulinase family | 939 |
| TC0194 | + | + | − | + | Conserved hypothetical protein | 146 |
| TC0199 | + | + | − | − | Hypothetical protein | 215 |
| TC0210 | + | + | − | + | Serine protease, HtrA/DegQ/DegS family | 497 |
| TC0212 | − | + | − | − | Conserved hypothetical protein | 425 |
| TC0224 | + | + | − | − | Phenylalanyl-tRNA synthetase | 341 |
| TC0230 | + | + | + | + | Polyribonucleotide nucleotidyltransferase | 693 |
| TC0232 | + | + | − | − | Cytidine/deoxycytidylate deaminase family protein | 157 |
| TC0234 | + | + | − | − | Conserved hypothetical protein | 234 |
| TC0236 | + | + | − | − | Conserved hypothetical protein | 170 |
| TC0244 | + | + | + | + | Fumarate hydratase | 463 |
| TC0248 | + | + | − | − | Conserved hypothetical protein | 601 |
| TC0250 | + | + | − | − | Conserved hypothetical protein | 497 |
| TC0251 | + | + | + | − | Conserved hypothetical protein | 508 |
| TC0253 | + | − | + | + | Conserved hypothetical protein | 172 |
| TC0256 | − | + | − | − | Conserved hypothetical protein | 328 |
| TC0258 | + | + | − | − | Hypothetical protein | 335 |
| TC0265 | + | + | − | − | Hypothetical protein | 84 |
| TC0268 | + | + | + | + | Hypothetical protein | 614 |
| TC0269 | + | + | − | − | Conserved hypothetical protein | 89 |
| TC0279 | + | + | − | − | Conserved hypothetical protein | 418 |
| TC0285 | + | + | − | + | Conserved hypothetical protein | 446 |
| TC0290 | − | + | − | − | Conserved hypothetical protein | 247 |
| TC0300 | + | + | − | − | Conserved hypothetical protein | 100 |
| TC0309 | + | + | − | − | dCTP deaminase | 190 |
| TC0311 | + | + | − | − | Conserved hypothetical protein | 286 |
| TC0319 | + | + | − | − | Hypothetical protein | 434 |
| TC0323 | + | + | − | − | Conserved hypothetical protein | 148 |
| TC0328 | + | + | − | − | Conserved hypothetical protein | 374 |
| TC0331 | + | + | − | − | RNA polymerase sigma factor, sigma-70 family | 253 |
| TC0335 | + | + | − | − | ADP, ATP carrier protein | 529 |
| TC0336 | + | + | + | − | Conserved hypothetical protein | 158 |
| TC0344 | + | + | − | − | Conserved hypothetical protein | 619 |
| TC0347 | + | + | − | − | DNA polymerase III, beta subunit | 366 |
| TC0351 | + | + | − | + | Conserved hypothetical protein | 157 |
| TC0352 | + | + | − | + | Hypothetical protein | 133 |
| TC0363 | + | + | − | + | Type III secretion chaperone, SycE | 146 |
| TC0372 | + | + | − | + | N utilization substance protein A | 434 |
| TC0373 | + | + | + | + | Ribosomal protein S1 | 536 |
| TC0381 | + | + | − | − | Hypothetical protein | 650 |
| TC0386 | + | + | + | + | 60-kDa chaperonin | 544 |
| TC0387 | + | + | + | + | 10-kDa chaperonin | 102 |
| TC0388 | + | + | − | − | Oligoendopeptidase F | 608 |
| TC0389 | + | + | + | − | ATP-dependent Clp protease, subunit B | 867 |
| TC0390 | + | + | + | + | Conserved hypothetical protein | 491 |
| TC0392 | + | + | − | − | Hypothetical protein | 143 |
| TC0396 | + | + | − | − | Inclusion membrane protein | 276 |
| TC0399 | + | + | + | − | Acetyl-coenzyme A carboxyl carrier | 163 |
| TC0410 | + | + | − | − | Conserved hypothetical protein | 266 |
| TC0420 | + | + | − | − | Conserved hypothetical protein | 279 |
| TC0422 | + | + | − | − | Conserved hypothetical protein | 614 |
| TC0425 | + | + | − | + | Monooxygenase related protein | 506 |
| TC0437 | + | + | − | − | Adherence factor | 3,255 |
| TC0438 | + | + | + | + | Adherence factor | 3,335 |
| TC0439 | + | + | + | + | Adherence factor | 3,225 |
| TC0446 | + | + | − | − | Peptide ABC transporter, periplasmic peptide binding protein | 527 |
| TC0448 | − | + | − | − | Conserved hypothetical protein | 135 |
| TC0449 | + | + | + | − | Conserved hypothetical protein | 237 |
| TC0458 | − | + | − | − | Glucosamine-6-phosphate isomerase, putative | 256 |
| TC0473 | + | + | − | + | Peptide ABC transporter, permease protein | 287 |
| TC0478 | + | − | − | + | Conserved hypothetical protein | 272 |
| TC0479 | − | + | − | + | Pyrophosphate-fructose 6 phosphotransferase | 548 |
| TC0491 | − | + | − | − | Stationary-phase survival protein, SurE | 291 |
| TC0500 | + | + | − | − | Hypothetical protein | 214 |
| TC0503 | + | + | − | − | Inclusion membrane protein B | 123 |
| TC0504 | + | + | − | − | Hypothetical protein | 177 |
| TC0512 | + | + | − | − | Outer membrane protein, putative | 792 |
| TC0519 | + | + | − | − | Glycogen phosphorylase | 813 |
| TC0521 | + | + | + | + | Chromosomal replication initiator protein, DnaA | 456 |
| TC0522 | + | + | − | − | Inner membrane protein, putative | 787 |
| TC0527 | + | + | − | − | Conserved hypothetical protein | 412 |
| TC0536 | − | + | − | − | Acetyl-coenzyme A carboxylase carboxyl transferase, alpha subunit | 324 |
| TC0539 | + | + | − | − | N-Acetylmuramoyl-l-alanine amidase, putative | 268 |
| TC0551 | + | + | + | + | NADH:ubiquinone oxidoreductase, gamma subunit, putative | 318 |
| TC0557 | + | + | − | − | Phospholipase D family protein | 474 |
| TC0561 | + | + | − | − | Conserved hypothetical protein | 558 |
| TC0574 | + | + | − | − | Hypothetical protein | 127 |
| TC0582 | + | + | − | + | ATP synthase, subunit A | 591 |
| TC0588 | + | + | − | − | DNA-directed RNA polymerase, beta subunit | 1,396 |
| TC0589 | + | + | − | − | DNA-directed RNA polymerase | 1,252 |
| TC0590 | + | + | − | − | Ribosomal protein L7/L12 | 130 |
| TC0596 | + | − | − | − | Translation elongation factor Tu | 399 |
| TC0619 | + | + | − | − | DnaJ protein | 392 |
| TC0623 | + | + | − | + | Protease, Lon family | 819 |
| TC0629 | − | + | − | + | Conserved hypothetical protein | 566 |
| TC0631 | − | + | + | + | Conserved hypothetical protein | 102 |
| TC0632 | + | + | − | − | Polypeptide deformylase | 181 |
| TC0638 | − | + | − | − | BioY family protein | 196 |
| TC0652 | + | + | + | + | Conserved hypothetical protein | 195 |
| TC0660 | + | + | + | + | Amino acid ABC transporter | 259 |
| TC0661 | + | + | − | + | Phospho-2-dehydro-3-deoxyheptonate aldolase, putative | 279 |
| TC0664 | + | + | − | − | HIT family protein | 126 |
| TC0675 | + | − | + | − | DnaK protein | 658 |
| TC0677 | + | + | − | + | Conserved hypothetical protein | 254 |
| TC0680 | + | + | + | + | 2-Oxo acid dehydrogenase, E2 component, lipoamide acyltransferase | 410 |
| TC0688 | − | + | − | − | Signal peptidase II | 167 |
| TC0691 | + | + | − | + | Poly(A) polymerase family protein | 425 |
| TC0701 | + | + | − | − | Ribosomal protein L21, RplU | 107 |
| TC0705 | + | + | − | − | Conserved hypothetical protein | 161 |
| TC0706 | − | + | + | + | Hemolysin, putative | 374 |
| TC0709 | + | + | + | + | Hypothetical protein | 71 |
| TC0721 | + | + | − | − | Translation elongation factor | 694 |
| TC0725 | + | + | − | − | Tail specific protease precursor, putative | 649 |
| TC0726 | + | + | − | − | Sulfur-rich protein | 152 |
| TC0727 | + | + | + | + | 60-kDa outer membrane protein | 554 |
| TC0728 | − | + | − | − | Cysteine-rich outer membrane protein 3 | 88 |
| TC0733 | − | + | − | − | SecDF protein, putative | 1,400 |
| TC0738 | + | + | − | + | 1-Acyl-sn-glycerol-3-phosphate acyltransferase, putative | 216 |
| TC0740 | − | + | − | − | UDP-N-acetylglucosamine 1-carboxyvinyltransferase | 442 |
| TC0746 | − | + | − | − | Conserved hypothetical protein | 329 |
| TC0755 | + | + | − | − | Conserved hypothetical protein | 234 |
| TC0756 | − | + | − | + | Conserved hypothetical protein | 209 |
| TC0759 | + | + | − | − | Conserved hypothetical protein | 300 |
| TC0760 | + | − | − | − | Phenylalanyl-tRNA synthetase | 790 |
| TC0763 | + | + | − | − | Peptide ABC transporter, permease protein, putative | 578 |
| TC0773 | + | + | − | − | Amino acid ABC transporter | 263 |
| TC0781 | + | + | + | + | Protease IV, putative | 332 |
| TC0787 | + | − | − | − | Nucleoside 2P kinase | 141 |
| TC0788 | + | − | − | − | Holliday junction DNA helicase, RuvA | 200 |
| TC0790 | + | + | + | + | Conserved hypothetical protein | 185 |
| TC0794 | + | + | − | − | DNA-directed RNA polymerase | 394 |
| TC0797 | − | + | − | − | Preprotein translocase, SecY subunit | 457 |
| TC0799 | + | + | − | − | Ribosomal protein S5 | 165 |
| TC0802 | + | + | − | − | Ribosomal protein S8 | 133 |
| TC0816 | + | + | − | − | Conserved hypothetical protein | 293 |
| TC0817 | − | + | − | − | Methionyl-tRNA formyltransferase | 316 |
| TC0819 | + | − | + | + | 3R-hydroxymyristol-acyl carrier | 153 |
| TC0822 | + | + | − | − | Cytosolic acyl-coenzyme A thioester hydrolase family protein | 159 |
| TC0828 | + | + | − | + | Peptidyl-prolyl cis-trans isomerase, Mip | 261 |
| TC0833 | + | + | − | + | Uracil phosphoribosyltransferase | 303 |
| TC0835 | − | + | − | − | Conserved hypothetical protein | 318 |
| TC0836 | + | + | − | − | Conserved hypothetical protein | 199 |
| TC0838 | − | + | − | + | Conserved hypothetical protein | 140 |
| TC0848 | + | + | + | - | Type III secretion protein SctJ | 328 |
| TC0850 | + | + | + | + | Type III secretion translocase, SctL | 235 |
| TC0854 | + | + | − | − | Conserved hypothetical protein | 147 |
| TC0855 | − | + | − | − | Conserved hypothetical protein | 330 |
| TC0861 | + | + | + | + | General secretion pathway protein D | 759 |
| TC0884 | + | + | − | − | Thiol:disulfide interchange protein, DsbD, putative | 692 |
| TC0892 | + | − | − | − | Antioxidant, AhpC/Tsa family | 195 |
| TC0901 | − | + | − | − | Conserved hypothetical protein | 243 |
| TC0903 | + | + | − | + | 2-Amino-4-hydroxy-6-hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase | 450 |
| TC0909 | + | + | − | + | Conserved hypothetical protein | 875 |
| TC0913 | + | + | − | − | Conserved hypothetical protein | 548 |
| TC0919 | + | + | − | − | Transcriptional regulator | 227 |
| TCA05 | + | + | − | − | Virulence protein pGP4-D | 102 |
| TCA07 | − | + | − | − | Virulence protein pGP6-D | 246 |
a
aa, amino acids.