FIG. 3.

Sensitivity of wild-type P. aeruginosa KU2 and the lepA mutant to iron limitation in hemoglobin medium. (A) Effect of iron limitation by 2,2′-dipyridyl on growth. (B) Restoration of growth by the addition of FeCl₃. The growth rate of each P. aeruginosa strain was monitored sequentially (A) or after 48 h of incubation (B) by measuring the optical density at 600 nm. Values represent the mean results from duplicate (A) or triplicate (B) determinations. Error bars indicate the plus-or-minus standard deviation. The data from a representative experiment are presented, and similar results were obtained in three independent experiments.