FIG. 5.

Cytotoxicity of wild-type P. aeruginosa KU2 and the lepA and plcH mutants for human cell lines. THP-1 or Jurkat cells (5 × 10⁴ cells) were cocultured with each P. aeruginosa strain (5 × 10⁴ CFU) for 4 h. The cytotoxicity of each P. aeruginosa strain was evaluated by an assay measuring LDH release from the cells. Values represent the mean results ± standard deviations from triplicate determinations. The data from a representative experiment are presented, and similar results were obtained in three independent experiments. The difference between KU2ΔlepA and KU2ΔplcH was significant with a P value of <0.05. The differences of all the other pairings were significant with P values of <0.01.