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Secondary structure of BAR and BAR-15. (A) Secondary structure predictions of BAR and BAR-15 indicate that substitution of Gln for Pro1171 increases the α-helical content of BAR-15. (B) Circular dichroism spectra of BAR (triangles) and BAR-15 (squares) show increased negative ellipticity for BAR-15 at the λ of 222 nm, consistent with increased α-helical structure.
