FIG. 3.

In vitro bactericidal peptide activity of C57BL/6-derived α-defensins. Exponentially growing cells of the Salmonella enterica serovar Typhimurium phoPΔ strain (A), Escherichia coli ML35 (B), Staphylococcus aureus (C), and Listeria monocytogenes (D) were exposed to the peptide concentrations shown at 37°C in 50 ml 10 mM PIPES buffer supplemented with 1% TSB for 1 h. Symbols: •, Crp20; ○ Crp4; ▿, Crp27; and ▾, Crp24. Following peptide exposure, the bacteria were plated on Trypticase soy agar and incubated overnight at 37°C, and bacterial cell survival was determined by counting the CFU/ml at each peptide concentration. The panels are representative of three independent experiments in each case. Values at or below 1 × 10³ CFU/ml signify that no colonies were detected.