TABLE 3.
Microbe | OCl− concn (μM) | No. of CFU/mla |
Hemoglobin contenta,b |
||
---|---|---|---|---|---|
Without RBCs | With RBCs (107) | Supernatant | Pellet | ||
E. coli | 0.0000 | 1,700,000 | 1,500,000 | 0.0 | 0.9 |
6,300.0000 | 0 | 0 | 0.0 | 0.0 | |
630.0000 | 0 | 1,400,000 | 0.3 | 0.1 | |
63.0000 | 0 | 1,400,000 | 0.7 | 0.0 | |
6.3000 | 0 | 1,700,000 | 0.0 | 1.0 | |
0.6300 | 1,600,000 | 1,500,000 | 0.0 | 0.9 | |
0.0630 | 1,500,000 | 1,500,000 | 0.0 | 1.0 | |
0.0063 | 1,500,000 | 1,600,000 | 0.0 | 1.0 | |
0.0006 | 1,400,000 | 1,600,000 | 0.0 | 1.0 | |
S. aureus | 0.0000 | 1,500,000 | 1,400,000 | 0.0 | 1.0 |
6,300.0000 | 0 | 0 | 0.0 | 0.0 | |
630.0000 | 0 | 71,000 | 0.3 | 0.1 | |
63.0000 | 0 | 1,200,000 | 1.0 | 0.0 | |
6.3000 | 0 | 1,500,000 | 0.0 | 1.0 | |
0.6300 | 1,300,000 | 1,400,000 | 0.0 | 0.9 | |
0.0630 | 1,400,000 | 1,500,000 | 0.0 | 1.0 | |
0.0063 | 1,300,000 | 1,200,000 | 0.0 | 1.0 | |
0.0006 | 1,500,000 | 1,300,000 | 0.0 | 1.0 | |
P. aeruginosa | 0.0000 | 1,800,000 | 2,700,000 | 0.1 | 0.9 |
6,300.0000 | 0 | 0 | 0.0 | 0.0 | |
630.0000 | 0 | 2,000,000 | 1.0 | 0.0 | |
63.0000 | 0 | 2,300,000 | 0.0 | 1.0 | |
6.3000 | 400 | 2,100,000 | 0.0 | 1.0 | |
0.6300 | 5,000 | 2,300,000 | 0.1 | 0.9 | |
0.0630 | 2,100 | 2,300,000 | 0.0 | 1.0 | |
0.0063 | 1,200 | 2,900,000 | 0.1 | 0.9 | |
0.0006 | 2,100,000 | 2,500,000 | 0.0 | 0.9 | |
C. albicans | 0.0000 | 360,000 | 340,000 | 0.1 | 1.0 |
6,300.0000 | 0 | 0 | 0.0 | 0.0 | |
630.0000 | 0 | 350,000 | 0.2 | 0.0 | |
63.0000 | 0 | 350,000 | 0.9 | 0.1 | |
6.3000 | 240,000 | 250,000 | 0.1 | 0.9 | |
0.6300 | 250,000 | 310,000 | 0.1 | 0.7 | |
0.0630 | 280,000 | 360,000 | 0.1 | 0.8 | |
0.0063 | 240,000 | 340,000 | 0.1 | 0.9 | |
0.0006 | 290,000 | 290,000 | 0.1 | 0.9 |
Values in boldface are less than 3 SDs from the expected mean and therefore indicate significant killing or significant hemolysis.
RBC damage was assessed by measuring the hemoglobin (Hgb) retained in the pelleted intact RBCs, lost to the medium as a consequence of hemolysis, or completely destroyed by oxidation. Hgb was calculated as described in footnote b of Table 1.