TABLE 3.

Direct microbicidal action of hypochlorite in the absence and presence of human erythrocytes

Microbe	OCl− concn (μM)	No. of CFU/mla		Hemoglobin contenta,b
		Without RBCs	With RBCs (107)	Supernatant	Pellet
E. coli	0.0000	1,700,000	1,500,000	0.0	0.9
	6,300.0000	0	0	0.0	0.0
	630.0000	0	1,400,000	0.3	0.1
	63.0000	0	1,400,000	0.7	0.0
	6.3000	0	1,700,000	0.0	1.0
	0.6300	1,600,000	1,500,000	0.0	0.9
	0.0630	1,500,000	1,500,000	0.0	1.0
	0.0063	1,500,000	1,600,000	0.0	1.0
	0.0006	1,400,000	1,600,000	0.0	1.0
S. aureus	0.0000	1,500,000	1,400,000	0.0	1.0
	6,300.0000	0	0	0.0	0.0
	630.0000	0	71,000	0.3	0.1
	63.0000	0	1,200,000	1.0	0.0
	6.3000	0	1,500,000	0.0	1.0
	0.6300	1,300,000	1,400,000	0.0	0.9
	0.0630	1,400,000	1,500,000	0.0	1.0
	0.0063	1,300,000	1,200,000	0.0	1.0
	0.0006	1,500,000	1,300,000	0.0	1.0
P. aeruginosa	0.0000	1,800,000	2,700,000	0.1	0.9
	6,300.0000	0	0	0.0	0.0
	630.0000	0	2,000,000	1.0	0.0
	63.0000	0	2,300,000	0.0	1.0
	6.3000	400	2,100,000	0.0	1.0
	0.6300	5,000	2,300,000	0.1	0.9
	0.0630	2,100	2,300,000	0.0	1.0
	0.0063	1,200	2,900,000	0.1	0.9
	0.0006	2,100,000	2,500,000	0.0	0.9
C. albicans	0.0000	360,000	340,000	0.1	1.0
	6,300.0000	0	0	0.0	0.0
	630.0000	0	350,000	0.2	0.0
	63.0000	0	350,000	0.9	0.1
	6.3000	240,000	250,000	0.1	0.9
	0.6300	250,000	310,000	0.1	0.7
	0.0630	280,000	360,000	0.1	0.8
	0.0063	240,000	340,000	0.1	0.9
	0.0006	290,000	290,000	0.1	0.9

^aValues in boldface are less than 3 SDs from the expected mean and therefore indicate significant killing or significant hemolysis.

^bRBC damage was assessed by measuring the hemoglobin (Hgb) retained in the pelleted intact RBCs, lost to the medium as a consequence of hemolysis, or completely destroyed by oxidation. Hgb was calculated as described in footnote b of Table 1.