TABLE 4.
Microbe added to S. sanguinis culturea | MPO concn (nM) | No. of CFU/mlb |
Hemoglobin contentc |
||
---|---|---|---|---|---|
Without RBCs | With RBCs (107) | Supernatant | Pellet | ||
E. coli | 0.0 | 2,000,000 | 2,600,000 | 0.0 | 1.0 |
50.0 | 0 | 0 | 0.0 | 1.0 | |
16.7 | 0 | 0 | 0.0 | 1.0 | |
5.6 | 0 | 10,000 | 0.0 | 1.0 | |
1.9 | 10,000 | 930,000 | 0.0 | 1.1 | |
0.6 | 650,000 | 2,600,000 | 0.0 | 1.0 | |
0.2 | 1,900,000 | 2,500,000 | 0.0 | 1.0 | |
S. aureus | 0.0 | 1,600,000 | 1,800,000 | 0.0 | 1.0 |
50.0 | 0 | 8,000 | 0.0 | 0.9 | |
16.7 | 0 | 740,000 | 0.0 | 1.0 | |
5.6 | 820,000 | 930,000 | 0.0 | 1.0 | |
1.9 | 430,000 | 1,900,000 | 0.0 | 1.0 | |
0.6 | 1,600,000 | 1,700,000 | 0.1 | 1.0 | |
0.2 | 1,700,000 | 1,900,000 | 0.0 | 1.0 | |
P. aeruginosa | 0.0 | 2,000,000 | 3,400,000 | 0.0 | 1.0 |
50.0 | 0 | 0 | 0.1 | 1.0 | |
16.7 | 0 | 10,000 | 0.0 | 0.9 | |
5.6 | 0 | 7,200 | 0.0 | 0.9 | |
1.9 | 0 | 6,200 | 0.0 | 1.0 | |
0.6 | 0 | 520,000 | 0.1 | 1.0 | |
0.2 | 10,000 | 4,500,000 | 0.0 | 1.0 | |
C. albicans | 0.0 | 200,000 | 240,000 | 0.0 | 1.0 |
50.0 | 0 | 4,000 | 0.0 | 0.8 | |
16.7 | 0 | 250,000 | 0.0 | 0.9 | |
5.6 | 0 | 180,000 | 0.0 | 1.0 | |
1.9 | 0 | 240,000 | 0.0 | 1.0 | |
0.6 | 6,000 | 250,000 | 0.0 | 1.0 | |
0.2 | 160,000 | 210,000 | 0.0 | 1.0 |
Each suspension contained approximately 107 S. sanguinisbacteria plus the additional microbe, as indicated. Microbe concentration was estimated by the optical density measurement at 540 nm. No H2O2 was added.
Values in boldface are less than 3 SDs from the expected mean and therefore indicate significant killing.
RBC damage was assessed by measuring the hemoglobin (Hgb) retained in the pelleted intact RBCs, lost to the medium as a consequence of hemolysis, or completely destroyed by oxidation as described in footnote b of Table 1.