FIG. 6.
Nigg is the predominant strain in the oviduct. (A) Oviducts were harvested from mice sacrificed on day 10 of infection. The bacterial load associated with the oviduct homogenates from each mouse was determined by a plaque assay. Plaques formed by Nigg were identified via immunofluorescent staining using anti-TCAO8 antibody. *, P < 0.05 for CM972 versus each group infected with Nigg (one-way ANOVA with multiple comparisons). The Nigg groups were not statistically significantly different from each other. (B to D) Genital tracts from mice in each group sacrificed on day 14 were scored for histopathology. Median pathology scores for acute inflammatory infiltrates (neutrophils) (B), chronic inflammatory infiltrates (lymphocytes and monocytes) (C), and oviduct dilatation (D) were significantly increased in mice infected with either 105 Nigg or 5 × 104 Nigg (Nigg ratio of 1:1) compared to the results obtained with mice in each of the other three groups. *, P < 0.05 by ANOVA on ranks with multiple-comparison test. Results for mice infected with 1:10 and 1:100 ratios of Nigg were not different from those seen with mice infected with CM972. Data represent the means ± standard deviations of the results obtained for each group of 5 mice in a single experiment. Boxes extend from the 25th to 75th percentiles, and whiskers indicate the 5th to 95th percentiles.