In vivo assessment of virulence. Bacterial growth in the lungs (A) and spleens (B) of C57BL/6 mice. Six- to 8-week-old mice were used, and they were infected via aerosolization with about 150 CFU of H37Rv (solid black line), H37Rv:ΔRD2 (solid gray line), H37Rv:ΔRD1 (dashed gray line), or BCG Pasteur (dashed black line). Data are the means ± standard errors of the means of 10 mice per strain from two separate infections for H37Rv and H37Rv:ΔRD2 and 4 mice per strain from one infection for H37Rv:ΔRD1 and BCG Pasteur. *, P < 0.05.