TABLE 2.

Oligonucleotide primers used in this study

Description	Sequence
Upstream of purH (5′); used to construct purH::Kan and ΔpurH mutants	5′-TGATAAACCGGTACCACGCGCTGTTGG-3′
Downstream of purH (3′); used to construct purH::Kan and ΔpurH mutants	5′-ATAATCTCTAGAAGTTTCGTACGCTGCAGTTG-3′
purH screening primer (5′)	5′-CAATGGGGGCAAAGCACTATGAG-3′
purH screening primer (3′)	5′-ATCGCTACTGTTACACCTTTACCA-3′
Upstream of purH (3′); used to construct ΔpurH mutant	5′-ACGCTTTTTCCCGGGTCCTTCACCCCTGGTTGTATG-3′
Downstream of purH (5′); used to construct ΔpurH mutant	5′-TTCAAACACCCCGGGAGGAAGTTTAAAAAGTCCGG-3′
Upstream of purD (5′); used to construct ΔpurD mutant	5′-ATTATTATGGTACCTTCTTTCT-3′
Upstream of purD (3′); used to construct ΔpurD mutant	5′-TGCTACACCCGGGTTTTCTACTTT-3′
Downstream of purD (5′); used to construct ΔpurD mutant	5′-TCGCCCGGGTTGAGAGTGATG-3′
Downstream of purD (3′); used to construct ΔpurD mutant	5′-TCTCTAGAATTAAAGCGTGAGT-3′
capC (5′)	5′-ACTCGTTTTTAATCAGCCCG-3′
capC (3′)	5′-GGTAACCCTTGTCTTTGAAT-3′
pagA (5′)	5′-GTGCATGCGTCGTTCTTTGA-3′
pagA (3′)	5′-GCCGCTATCCGCCTTTCTA-3′
purH (5′); used to construct ΔpurH + purH strain	5′-ATACAACCAGGGGTGAAGGTACCATGAAAAAGC-3′
purH (3′); used to construct ΔpurH + purH strain	5′-AAACTTCCGTTTAAACTGTTTGAAATGACGTACG-3′
Upstream of purH (3′); SmaI site used to construct ΔpurH + purD strain	5′-TGCACGCTTTTCCCGGGATCCTT-3′
pEO3-specific primer	5′-AAAAGTGCCACCTGACGTCTAAG-3′
purD (5′); used to construct ΔpurH + purD strain	5′-TTCCCGGGTGAAATATGAATGTT-3′
purD (3′); used to construct ΔpurD + purD and ΔpurH + purD strains	5′-GGCTCTAGAAAAAGGTAAAT-3′
Upstream of purD (5′); used to construct ΔpurD + purD strain	5′-GGTGGTACCCTCGTTCAA-3′
purD screening primer (5′)	5′-ATGCAGCGCTTAGTATCG-3′
purD screening primer (3′)	5′-TATTATTTTAGCCGCAGTTCTT-3′
Internal purD primer for RT-PCR (5′)	5′-CAAATACTGGCGGAATGG-3′
Internal purD primer for RT-PCR (3′)	5′-CTAGCCCTTTAATAATGTCACCTT-3′