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. 2010 Oct 22;193(1):163–176. doi: 10.1128/JB.00852-10

FIG. 2.

FIG. 2.

Effect of the shvR mutation on AHL activity and expression of QS genes. (A) AHL activity was measured using the biosensor A. tumefaciens A136(pCF218)(pMV26) in coculture with B. cenocepacia at 29°C. AHL activity was significantly increased from 22 to 26.5 h (log phase) and significantly decreased from 35 to 45 h (stationary phase) in K56-2ΔshvR(pUCP28T) compared to that in K56-2(pUCP28T) (P < 0.05, two-way ANOVA). Expression of cepI (B), cepR (C), or cciIR (D) was monitored using promoter::lux fusions (pCP300, pRM432, or pRM445, respectively) in K56-2 and K56-2ΔshvR in LB at 37°C. Expression is significantly increased in K56-2ΔshvR compared to that in K56-2 from 24.5 to 40.5 h for cepI, from 5.5 to 49.5 h for cepR, and from 19 to 49.5 h for cciIR (P < 0.001, two-way ANOVA). All values are the means ± SD of results from triplicate cultures and are representative of results from at least two individual trials.