TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequence (5′→3′) | Reference |
|---|---|---|
| 5′-XbaI-UP-BCAS0225 | TTTTTCTAGATGCGCATCGAATGCACACCG | This study |
| 3′-KpnI-UP-BCAS0225 | TTTTGGTACCCTTCTCAATCGCTTTGCC | This study |
| 5′-KpnI-DWN-BCAS0225 | TTTTGGTACCACGTGCTGCCGATGTATCGC | This study |
| 3′-SmaI-DWN-BCAS0225 | TTTTCCCGGGATCTTCCAGATTCACGTCCG | This study |
| XhoI-5′-PBCAS0225 | AGCTCTCGAGCGGATTCATCTTGACGGTCG | This study |
| PBCAS0225-3′-BamHI | AGCTGGATCCGGCAGACTTCTCAATCGCTT | This study |
| PgspC&Gfor1 | GGCTCGAGTGTCGAGACGATACAGCTTGAG | This study |
| PgspC&Grev1 | GGGGATCCCATATTTGCCATCCATTTGC | This study |
| PafcCfor1 | GGCTCGAGCGATTCGATCAGACGGTGAATG | This study |
| PafcCrev1 | GGGGATCCGACAAGCGATGAGGTGACG | This study |
| Fndh | GCGATCGGGCTGTACAAGTT | 53 |
| Rndh | AGTGGCTCAGCGACTGGAA | 53 |
| BCAS0220qRTfor2 | GAACCAGTTCTCGGTGTTCG | This study |
| BCAS0220qRTrev2 | GATCCAGTTGCTCATCGACA | This study |
| BCAS0215qRTfor1 | ATCCTGTCGATGCTGCTGAT | This study |
| BCAS0215qRTrev1 | TACGAGCAGGTCATCCAGTC | This study |
| BCAS0208qRTfor1 | ATCTGCAAGGCGTTCATCTC | This study |
| BCAS0208qRTrev1 | GTAGTTCGTGCCTTCCCAGA | This study |
| BCAS0204qRTfor1 | GCAATTGCAGAAGGTCGAGT | This study |
| BCAS0204qRTrev1 | CGACGATCTCGGATACACG | This study |
| pUCP28TflankMCSfor1 | CGACGTTGTAAAACGACGG | This study |
| pUCP28TflankMCSrev1 | GGAAACAGCTATGACCATGA | This study |