FIG. 2.

Sequence alignment of Cmi and YebF proteins. Amino acid sequences of YebF orthologues from Yersinia pestis CO92 (YERPE), Yersinia enterocolitica 8081 (YEREN), Escherichia coli (ECOLI), and Salmonella enterica serovar Typhimurium LT2 (SALTY) were aligned with the Cmi sequence using the ClustalW2 program (28). The 21-residue sequence signal of E. coli YebF that is removed following translocation of the protein to the periplasm and the 23-residue sequence of Cmi involved in attachment of the immunity protein to the membrane are boxed.